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Image Search Results
Journal: PLOS One
Article Title: Neural and endothelial cell-derived extracellular vesicles mediate Zika virus genome dissemination and productive infection in vivo
doi: 10.1371/journal.pone.0337609
Figure Lengend Snippet: Infection of all three cell types was confirmed by IF detection of the ZIKV C protein (green) at 48 hpi. Infected cells were identified through co-staining with cell-type specific markers (red). While all cultures demonstrated susceptibility to ZIKV infection, (A) neurons exhibited the most pronounced morphological alteration compared to uninfected controls. Notably, viral antigen is localized not only to perinuclear region but also to the axon hillock (arrow) and neurites (arrowhead). In contrast, (B) astrocytes and (C) MBECs maintained their typical cellular architecture despite infection. The highlighted square denotes a region of interest shown at higher magnification. Nuclei were counterstained with DAPI (blue). Scale bars represent 20 µm for neurons and MBECs, and 50 µm for astrocytes. Images are representative of two independent experiments, each comprising three replicates and eight fields analyzed per slide.
Article Snippet: Following this period, some wells were fixed with 4% PFA and processed by immunoperoxidase staining, using the
Techniques: Infection, Staining
Journal: PLOS One
Article Title: Neural and endothelial cell-derived extracellular vesicles mediate Zika virus genome dissemination and productive infection in vivo
doi: 10.1371/journal.pone.0337609
Figure Lengend Snippet: Immunoperoxidase staining for ZIKV E protein in A549 cell inoculated for 72 h with EVs or ZIKV. (A) Controls included non-infected cells (NIC), mock-treated cells (supernatants from uninfected C6/36HT cells), and supernatants of ZIKV-infected cells at a MOI of 0.1. (B) Brown peroxidase signal indicated productive infection in cells exposed to EVs-IC, while no detectable staining was observed in EVs-GlyR-treated cells. Representative images from three independent experiments, each performed in triplicate. Scale bars: 100 µm for controls and EVs-GlyR) and 50 µm for EVs-IC.
Article Snippet: Following this period, some wells were fixed with 4% PFA and processed by immunoperoxidase staining, using the
Techniques: Immunoperoxidase Staining, Infection, Staining